Selectin E/CD62e antibody

Catalog No. ABIN319451

The Mouse Monoclonal anti-Selectin E/CD62e antibody has been validated for WB, IP, FACS, IHC (fro) and EIA. It is suitable to detect Selectin E/CD62e in samples from Human and Pig.

Quick Overview for Selectin E/CD62e antibody (ABIN319451)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1-2B6

Product Details anti-Selectin E/CD62e Antibody

Specificity: This antibody recognises the CD62E and CD62P cell surface antigens. MCA883 inhibits neutrophil adhesion to E-selectin and to P-selectin. Although previously thought to recognise only human CD62E, recent data (5) shows that this antibody also recognises human CD62P binding to a common epitope on these members of the selectin family.

Cross-Reactivity (Details): Also reacts to: CD62P

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Immunogen: Human E-Selectin (ELAM-1)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: 1g/mL - 10g/mL. Positive Control: Human Tonsil. ELISA: 10g/mL. Western Blot: Non-reduced only, 10l of the suggested working dilution to label 10^6 cellsin 100 μL. Flow Cytometry: 10g/mL. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E / E-Selectin

Background: CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gene ID: 6401

NCBI Accession: NP_000441

UniProt: P16109

Pathways: Thromboxane A2 Receptor Signaling